首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠免疫球蛋白E(IgE)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的IgE含量。IgE是介导Ⅰ型超敏反应的关键抗体，在过敏反应中扮演重要角色。其研究机制主要围绕阻断IgE与其受体结合，抑制肥大细胞和嗜碱性粒细胞活化、脱颗粒，释放炎症介质，进而减轻过敏症状，为过敏性疾病治疗提供新方向。试剂盒检测范围为1.56 ng/mL-100 ng/mL，该产品适用于科研领域中小鼠过敏模型构建、免疫调节机制研究、抗过敏药物开发等场景，特别在评估实验动物体内IgE水平变化、免疫应答动态监测等实验中具有高特异性和稳定性。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IgE
种属：Mus musculus (Mouse)
样本类型：serum, plasma
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IgE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 89-95 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 99-105 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 87-96 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 94-101 | |||||
回收率：
| The recovery of mouse IgE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 91-97 | ||||
| EDTA plasma (n=4) | 95 | 91-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1002.4352.3282.3822.245501.7041.6841.6941.557251.1821.1661.1741.03712.50.5770.5900.5840.4476.250.4000.4100.4050.2683.120.3070.2920.3000.1631.560.2580.2480.2530.11600.1380.1360.137 | ||||||
货期：3-5 working days
×
