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产品描述：鸡胰岛素样生长因子1(IGF-1)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IGF1含量。IGF1即胰岛素样生长因子1，在细胞的生长、增殖和分化等过程中扮演重要角色。研究机制方面，它主要通过与IGF1受体结合，激活下游信号通路，影响细胞的存活、代谢等。其异常表达与多种疾病如肿瘤、糖尿病等密切相关，是相关研究的重要靶点。试剂盒检测范围为125 pg/mL-2000 pg/mL，该产品可广泛应用于家禽生长发育机制研究、营养代谢实验、繁殖生理学分析以及激素调控相关的基础科研领域，为实验室提供稳定可靠的检测工具。试剂盒配套包含标准品、检测板、生物素标记物及显色试剂等完整组分，操作流程标准化，适用于生命科学、动物医学等领域的科研实验需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IGF1 ELISA Kit; Insulin-like growth factor I ELISA Kit; IGF-I ELISA Kit; Somatomedin ELISA Kit
缩写：IGF1
Uniprot No.：P18254
种属：Gallus gallus (Chicken)
样本类型：serum, plasma, tissue homogenates
检测范围：125 pg/mL-2000 pg/mL
灵敏度：125 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-96 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-105 | |||||
| 1:4 | Average % | 87 | ||||
| Range % | 85-90 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 93-99 | |||||
回收率：
| The recovery of chicken IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-94 | ||||
| EDTA plasma (n=4) | 95 | 90-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 20000.1830.1940.189 10000.3110.3330.322 5000.5260.5000.513 2501.0241.0281.026 1252.1072.1202.114 | ||||||
货期：3-5 working days
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