Pig insulin-like growth factors-1,IGF-1 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E06829p

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：猪胰岛素样生长因子1(IGF-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、细胞裂解物样本中的IGF1含量。IGF1即胰岛素样生长因子1，在人体生长发育、细胞增殖分化等过程中起着关键作用。研究机制方面，它主要通过与IGF1受体结合，激活下游信号通路，影响细胞的生长、存活和代谢，在肿瘤、生长发育异常等疾病研究中受广泛关注。试剂盒检测范围为6.25 ng/mL-400 ng/mL，适用于猪模型相关的基础研究，例如生长调控机制探索、营养代谢实验、肌肉发育分析或疾病模型中IGF - 1信号通路的动态监测；为科研人员在体外实验、动物生理研究及细胞功能分析等领域提供可靠工具，满足实验室对IGF - 1蛋白定量检测的多样化需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IGF1 ELISA Kit; Insulin-like growth factor I ELISA Kit; IGF-I ELISA Kit; Somatomedin ELISA Kit
缩写：IGF1
Uniprot No.：P16545
种属：Sus scrofa (Pig)
样本类型：serum, plasma, cell culture supernates, tissue homogenates, cell lysates
检测范围：6.25 ng/mL-400 ng/mL
灵敏度：1.56 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-98
1:2	Average %	95
	Range %	90-104
1:4	Average %	97
	Range %	92-110
1:8	Average %	95
	Range %	82-100

回收率：

The recovery of pig IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	85-108
EDTA plasma (n=4)	95	91-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/mlOD1OD2AverageCorrected 4002.8242.8152.8202.676 2002.3622.3462.3542.210 1001.4691.4891.4791.335 500.7090.6840.6970.553 250.4160.4250.4210.277 12.50.2510.2620.2570.113 6.250.1990.1920.1960.052 00.1460.1420.144