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产品描述：兔子胰岛素样生长因子1(IGF-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IGF1含量。IGF1即胰岛素样生长因子 1 ，在人体生长发育、细胞增殖分化等生理过程中起重要作用。其研究机制主要聚焦于它与受体结合后激活下游信号通路，调控细胞功能。异常表达与多种疾病如肿瘤、糖尿病等相关，是药物研发的潜在靶点 。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于兔类动物模型的代谢研究、生长发育评估及与IGF - 1相关的疾病机制探索，如糖尿病、肌肉萎缩等病理状态分析本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IGF1 ELISA Kit; IGF-1Insulin-like growth factor I ELISA Kit; IGF-I ELISA Kit; Somatomedin ELISA Kit
缩写：IGF1
Uniprot No.：Q95222
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 80-100 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 92-115 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of rabbit IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-98 | ||||
| EDTA plasma (n=4) | 90 | 85-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.7152.7252.7202.60552.0352.0022.0191.9042.50.9891.0781.0340.9191.250.5910.6020.5970.4820.6250.3420.3520.3470.2320.3120.2010.2120.2070.0920.1560.1750.1850.1800.06500.1110.1190.115 | ||||||
货期：3-5 working days
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