| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SIX1 |
| Uniprot No | Q15475 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-284aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMSMLPS FGFTQEQVAC VCEVLQQGGN LERLGRFLWS LPACDHLHKN ESVLKAKAVV AFHRGNFREL YKILESHQFS PHNHPKLQQL WLKAHYVEAE KLRGRPLGAV GKYRVRRKFP LPRTIWDGEE TSYCFKEKSR GVLREWYAHN PYPSPREKRE LAEATGLTTT QVSNWFKNRR QRDRAAEAKE RENTENNNSS SNKQNQLSPL EGGKPLMSSS EEEFSPPQSP DQNSVLLLQG NMGHARSSNY SLPGLTASQP SHGLQTHQHQ LQDSLLGPLT SSLVDLGS |
| 预测分子量 | 35 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SIX1重组蛋白的3篇示例参考文献(文献信息为示例性质，具体内容需通过学术数据库验证)：
1. **《SIX1重组蛋白在乳腺癌转移中的功能研究》**
- 作者：Smith A, et al.
- 摘要：研究通过体外重组表达SIX1蛋白，发现其通过激活TGF-β信号通路促进乳腺癌细胞侵袭和转移，为靶向SIX1的癌症治疗提供依据。
2. **《重组SIX1蛋白的制备及其在胚胎发育中的作用》**
- 作者：Li X, et al.
- 摘要：利用大肠杆菌系统高效表达并纯化SIX1重组蛋白，证实其通过与Eya2蛋白互作调控胚胎肢体发育相关基因的表达。
3. **《SIX1重组蛋白与Wnt信号通路的相互作用机制》**
- 作者：Wang Y, et al.
- 摘要：通过体外结合实验和细胞模型，揭示SIX1重组蛋白通过增强β-catenin的核转导促进结肠癌细胞增殖，提示其在肿瘤发生中的潜在作用。
**提示**：建议通过PubMed、Google Scholar或Web of Science搜索关键词"recombinant SIX1 protein"或"SIX1 overexpression"获取最新文献。真实文献可能涉及SIX1在癌症、发育或代谢疾病中的重组表达及功能验证研究。
**Background of SIX1 Recombinant Protein**
The SIX1 (Sine oculis homeobox homolog 1) protein is a member of the SIX family of homeodomain transcription factors, which play critical roles in embryonic development, organogenesis, and tissue homeostasis. SIX1 is evolutionarily conserved and regulates processes such as cell proliferation, differentiation, and migration by binding to DNA via its homeodomain and interacting with cofactors like EYA (Eyes Absent) proteins. It is essential for the development of multiple organs, including the kidneys, muscles, and inner ear.
In cancer biology, SIX1 is often implicated in tumorigenesis and metastasis. Its aberrant overexpression has been linked to various cancers (e.g., breast, hepatocellular, and colorectal cancers), where it promotes epithelial-mesenchymal transition (EMT), invasiveness, and chemoresistance. SIX1 reprograms cellular metabolism and modulates signaling pathways like Wnt/β-catenin and TGF-β, contributing to oncogenic progression.
Recombinant SIX1 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, biologically active forms for research. This protein typically retains functional domains, including the DNA-binding homeodomain and the Six domain, enabling studies on its interaction networks, DNA-binding properties, and regulatory mechanisms. Researchers use recombinant SIX1 to investigate its role in developmental models, cancer cell lines, or regenerative medicine, as well as to screen inhibitors targeting its oncogenic activity.
Despite its significance, SIX1's dual role in development and disease underscores the need for precise experimental models to dissect context-dependent functions. Recombinant SIX1 thus serves as a vital tool for unraveling its mechanistic contributions to both normal physiology and pathology.
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