首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠胰岛素样生长因子2(IGF-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的IGF2含量。IGF2（胰岛素样生长因子2）是一种重要的促胚胎生长因子，通过自分泌或旁分泌方式作用于细胞表面的IGFⅡ受体，调节胚胎及滋养叶的生长发育。其表达受基因组印记调控，母源等位基因印记而父源等位基因表达。IGF2在细胞增殖、迁移、分化和存活等过程中起关键作用，功能失调会导致多种生长障碍。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于胚胎发育机制研究、代谢调控实验、肿瘤细胞增殖分析等科研场景，支持科研人员高效开展离体组织分析、细胞模型研究等基础实验，为生长发育调控及疾病机制探索提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Igf2 ELISA Kit; Igf-2Insulin-like growth factor II ELISA Kit; IGF-II ELISA Kit; Multiplication-stimulating polypeptide) [Cleaved into: Insulin-like growth factor II; Preptin] ELISA Kit
缩写：IGF2
Uniprot No.：P09535
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IGF-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:100 | Average % | 88 | |
| Range % | 84-97 | ||
| 1:200 | Average % | 96 | |
| Range % | 91-102 | ||
| 1:400 | Average % | 84 | |
| Range % | 81-88 | ||
| 1:800 | Average % | 92 | |
| Range % | 88-97 |
回收率：
| The recovery of mouse IGF-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 95-106 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 20002.6372.7742.7062.554 10002.3432.4512.3972.245 5001.8371.9351.8861.734 2501.1031.1901.1470.995 1250.6100.6320.6210.469 62.50.3740.3550.3650.213 31.250.2440.2480.2460.094 00.1500.1530.152 | ||||||
货期：3-5 working days
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