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产品描述：羊胰岛素样生长因子II(IGF2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IGF2含量。IGF2（胰岛素样生长因子2）是一种重要的促有丝分裂细胞因子，与IGF受体结合，调节多种组织生长。其研究机制涉及IGF2蛋白的分泌、转运和信号传导，对细胞增殖、迁移、分化和存活等过程起关键作用。IGF2的异常表达与多种生长障碍和肿瘤相关。试剂盒检测范围为15.6 ng/mL-1000 ng/mL，适用于农业科学、动物模型研究等领域，可助力探索IGF2在绵羊生长发育、营养代谢调控中的作用机制，或作为评估特定条件下生物体内IGF2动态变化的有效工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IGF2Insulin-like growth factor II ELISA Kit; IGF-II) [Cleaved into: Insulin-like growth factor II; Preptin] ELISA Kit
缩写：IGF2
Uniprot No.：P10764
种属：Ovis aries (Sheep)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 ng/mL-1000 ng/mL
灵敏度：3.9 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of sheep IGF2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 86-99 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 91-104 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 92-98 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 91-105 | |||||
回收率：
| The recovery of sheep IGF2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-97 | ||||
| EDTA plasma (n=4) | 96 | 90-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 10002.9022.9542.9282.751 5002.0312.1642.0981.921 2501.0891.0751.0820.905 1250.5920.5530.5730.396 62.50.3620.3590.3610.184 31.250.2780.2980.2880.111 15.60.2350.2130.2240.047 00.1760.1770.177 | ||||||
货期：3-5 working days
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