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产品描述：猪胰岛素样生长因子2(IGF-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的IGF2含量。IGF2即胰岛素样生长因子 2，是一种具有重要生物学功能的蛋白质。它参与胎儿生长发育、细胞增殖分化等过程。研究中，IGF2在肿瘤发生发展机制里受关注，与肿瘤细胞的生长、存活、侵袭等密切相关，是潜在的肿瘤治疗靶点。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于畜牧科学研究中动物生长性能评估、饲料效能分析、疾病状态下的代谢变化研究等场景，特别针对猪只生长发育监测、繁殖生理研究及应激反应机制探讨提供可靠检测方案本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IGF2Insulin-like growth factor II ELISA Kit; IGF-II) [Cleaved into: Insulin-like growth factor II; Preptin] ELISA Kit
缩写：IGF2
Uniprot No.：P23695
种属：Sus scrofa (Pig)
样本类型：serum, plasma
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IGF-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:20 | Average % | 89 | |||||
| Range % | 83-97 | ||||||
| 1:40 | Average % | 94 | |||||
| Range % | 90-103 | ||||||
| 1:80 | Average % | 92 | |||||
| Range % | 86-100 | ||||||
| 1:160 | Average % | 93 | |||||
| Range % | 87-101 | ||||||
回收率：
| The recovery of pig IGF-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 97 | 91-105 | |||||
| EDTA plasma (n=4) | 95 | 89-105 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected402.7542.7142.7342.565202.1442.1262.1351.966101.4391.4571.4481.27950.9250.9320.9290.7602.50.5250.5330.5290.3601.250.3810.3640.3730.2040.6250.2550.2490.2520.08300.1700.1680.169 | |||||||
货期：3-5 working days
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