首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述:人胰岛素样生长因子2 mRNA结合蛋白2(IGF2BP2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆、细胞裂解物样本中的IGF2BP2含量。IGF2BP2 是一种 RNA 结合蛋白。它可通过与特定 RNA 结合,调控基因表达和细胞活动。研究发现其与肿瘤发生发展有关,在肝癌、乳腺癌等癌症中表达异常,影响细胞增殖、凋亡等过程,有望成为癌症诊断标志物和治疗靶点。试剂盒检测范围为125 pg/mL-8000 pg/mL,适用于体外细胞模型构建、疾病动物模型评估以及临床前样本的分子标志物探索等科研场景,助力于肿瘤微环境研究、代谢性疾病机制解析及相关药物开发等基础研究领域。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:Hepatocellular carcinoma autoantigen p62 ELISA Kit; IF2B2_HUMAN ELISA Kit; IGF 2 mRNA binding protein 2 ELISA Kit; IGF II mRNA binding protein 2 ELISA Kit; IGF-II mRNA-binding protein 2 ELISA Kit; IGF2 mRNA-binding protein 2 ELISA Kit; Igf2bp2 ELISA Kit; IMP 2 ELISA Kit; IMP-2 ELISA Kit; IMP2 ELISA Kit; Insulin like growth factor 2 mRNA binding protein 2 ELISA Kit; Insulin-like growth factor 2 mRNA-binding protein 2 ELISA Kit; OTTHUMP00000082606 ELISA Kit; OTTHUMP00000082607 ELISA Kit; OTTHUMP00000082608 ELISA Kit; p62 ELISA Kit; VICKZ 2 ELISA Kit; VICKZ family member 2 ELISA Kit; VICKZ2 ELISA Kit
缩写:IGF2BP2
Uniprot No.:Q9Y6M1
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates, cell lysates
检测范围:125 pg/mL-8000 pg/mL
灵敏度:31.25 pg/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Epigenetics and Nuclear Signaling
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human IGF2BP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1 | Average % | 85 | |||||
Range % | 80-90 | ||||||
1:2 | Average % | 96 | |||||
Range % | 92-100 | ||||||
1:4 | Average % | 90 | |||||
Range % | 85-94 | ||||||
1:8 | Average % | 104 | |||||
Range % | 100-108 |
回收率:
The recovery of human IGF2BP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 89 | 84-93 | |||||
EDTA plasma (n=4) | 95 | 90-100 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
pg/mlOD1OD2AverageCorrected80002.1672.2492.2082.05140001.6491.7031.6761.51920001.1741.1141.1440.98710000.7910.7580.7750.6185000.4580.4720.4650.3082500.3350.3470.3410.1841250.2260.2360.2310.07400.1530.1600.157 |
货期:3-5 working days
×