| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SKP1 |
| Uniprot No | P63208 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-163aa |
| 氨基酸序列 | PSIKLQSSDGEIFEVDVEIAKQSVTIKTMLEDLGMDDEGDDDPVPLPNVNAAILKKVIQWCTHHKDDPPPPEDDENKEKRTDDIPVWDQEFLKVDQGTLFELILAANYLDIKGLLDVTCKTVANMIKGKTPEEIRKTFNIKNDFTEEEEAQVRKENQWCEEK |
| 预测分子量 | 45.5kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与SKP1重组蛋白相关的文献摘要示例(部分为模拟内容，实际文献需通过数据库验证)：
---
1. **文献名称**：*Recombinant SKP1 Expression in E. coli and Its Role in SCF Ubiquitin Ligase Assembly*
**作者**：Zhang Y, et al.
**摘要**：研究通过大肠杆菌系统高效表达并纯化重组人源SKP1蛋白，验证其与CUL1、F-box蛋白的相互作用，揭示其在SCF复合体组装中的结构基础，为泛素化机制研究提供工具。
2. **文献名称**：*Structural Analysis of SKP1-Cand1 Complex by Cryo-EM*
**作者**：Hao B, et al.
**摘要**：利用冷冻电镜解析SKP1与伴侣蛋白Cand1的复合物三维结构，阐明SKP1构象变化如何调控F-box蛋白的招募，为癌症靶向药物设计提供结构依据。
3. **文献名称**：*SKP1 Knockdown and Recombinant Rescue in Plant Defense Signaling*
**作者**：Li J, Wang X.
**摘要**：在拟南芥中通过基因沉默技术敲低SKP1导致抗病性下降，而外源添加重组SKP1蛋白可部分恢复防御反应，证明其在植物免疫信号通路中的保守功能。
---
注：以上文献信息为示例性质，实际引用时建议通过PubMed或Web of Science检索最新文献(关键词：SKP1 recombinant protein, SCF complex, ubiquitination)。
SKP1 (S-phase kinase-associated protein 1) is a core component of the SCF (SKP1-Cullin1-F-box protein) ubiquitin ligase complex, which plays a critical role in regulating protein degradation via the ubiquitin-proteasome system. As a key adaptor protein, SKP1 binds to F-box proteins to recruit specific substrates for ubiquitination, thereby controlling diverse cellular processes such as cell cycle progression, signal transduction, DNA repair, and apoptosis. Dysregulation of SKP1 or SCF complexes is implicated in cancers, neurodegenerative diseases, and immune disorders, making it a focal point in biomedical research.
Recombinant SKP1 proteins are engineered using heterologous expression systems (e.g., E. coli, mammalian, or insect cells) to produce purified, functional SKP1 for in vitro studies. These proteins retain the ability to interact with F-box partners and participate in substrate recognition, enabling researchers to dissect SCF complex mechanisms, screen for modulators, or study disease-related mutations. The recombinant form often includes affinity tags (e.g., His-tag, GST) for simplified purification and detection.
Studies leveraging recombinant SKP1 have advanced our understanding of its structural dynamics, including its role in substrate specificity and post-translational modifications like phosphorylation. Additionally, it serves as a tool in drug discovery, particularly in identifying inhibitors targeting oncogenic SCF ligases. Despite its conserved function, species-specific variations in SKP1 interactions highlight the importance of selecting appropriate recombinant systems for experimental relevance. Ongoing research continues to explore its therapeutic potential in diseases linked to ubiquitination pathways.
×
