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Mouse Insulin-like growth factor binding protein 1,IGFBP-1 ELISA Kit

  • 中文名： 小鼠胰岛素样生长因子结合蛋白1(IGFBP-1)ELISA Kit
货号: IPD-E04587m
Price： ￥2500
数量：
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  • 产品描述：小鼠胰岛素样生长因子结合蛋白1(IGFBP-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IGFBP1含量。IGFBP1即胰岛素样生长因子结合蛋白1，它在细胞生长、增殖等过程中起重要作用。其与糖代谢、肿瘤进展等密切相关。研究机制上，主要通过与胰岛素样生长因子结合，调节其活性与功能，进而影响相关生理和病理过程。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于肥胖、糖尿病等代谢疾病模型研究，或胚胎发育、肝脏再生等生理病理机制探索；适用于基础科研中动态监测IGFBP - 1表达水平，为代谢调控、生殖生物学及肝脏相关研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。

  • 别名：Igfbp1 ELISA Kit; Igfbp-1 ELISA Kit; Insulin-like growth factor-binding protein 1 ELISA Kit; IBP-1 ELISA Kit; IGF-binding protein 1 ELISA Kit; IGFBP-1 ELISA Kit

  • 缩写：IGFBP1

  • Uniprot No.：P47876

  • 种属：Mus musculus (Mouse)

  • 样本类型：serum, plasma, tissue homogenates

  • 检测范围：0.312 ng/mL-20 ng/mL

  • 灵敏度：0.078 ng/mL

  • 反应时间：1-5h

  • 样本体积：50-100ul

  • 检测波长：450 nm

  • 研究领域：Signal Transduction

  • 测定原理：quantitative

  • 测定方法：Sandwich

  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%



    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%



    Three samples of known concentration were tested in twenty assays to assess.










  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse IGFBP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    		SampleSerum(n=4)

    1:100Average %89

    Range %85-94

    1:200Average %102

    Range %96-107

    1:400Average %92

    Range %87-98

    1:800Average %94

    Range %89-98

  • 回收率：

    The recovery of mouse IGFBP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample TypeAverage % RecoveryRange

    Serum (n=5)9893-102

    EDTA plasma (n=4)10392-107

















  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/mlOD1OD2AverageCorrected202.1792.2342.2072.081101.8161.9431.8801.75451.3371.3451.3411.2152.50.8390.8460.8430.7171.250.4050.4120.4090.2830.6250.2780.2650.2720.1460.3120.1820.1890.1860.06000.1250.1260.126　







  • 货期：3-5 working days

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