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Human Insulin-like growth factor 2 mRNA-binding protein 3(IGF2BP3) ELISA kit

  • 中文名： 人胰岛素样生长因子2-mRNA结合蛋白3(IGF2BP3)ELISA kit
货号: IPD-EL011092HU
Price： ￥2500
数量：
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产品详情

  • 产品描述：人胰岛素样生长因子2-mRNA结合蛋白3(IGF2BP3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的IGF2BP3含量。IGF2BP3（胰岛素样生长因子2 mRNA结合蛋白3）是一种RNA结合蛋白，参与mRNA定位和翻译调控。其在胚胎发生和某些恶性肿瘤中表达，可作为骨肉瘤的独立预后因素。研究显示，IGF2BP3可能通过与MYC mRNA结合并增强其稳定性，促进细胞增殖、迁移和侵袭，影响肿瘤转化进程。试剂盒检测范围为37.5 pg/mL-2400 pg/mL，适用于基础科研领域中IGF2BP3蛋白表达的定量分析，可支持癌症相关研究的潜在生物标志物评估、分子机制探索或药物干预效果验证等实验需求。产品采用优化的抗体配对方案，确保检测的高特异性，同时兼容多种生物样本类型，为体外实验提供标准化检测工具，满足细胞模型、动物模型及临床前样本中IGF2BP3动态变化的定量研究需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。

  • 别名：Cancer/testis antigen 98 ELISA Kit; CT98 ELISA Kit; DKFZp686F1078 ELISA Kit; hKOC ELISA Kit; IF2B3_HUMAN ELISA Kit; IGF II mRNA binding protein 3 ELISA Kit; IGF-II mRNA-binding protein 3 ELISA Kit; IGF2 mRNA binding protein 3 ELISA Kit; IGF2 mRNA-binding protein 3 ELISA Kit; IGF2BP3 ELISA Kit; IMP 3 ELISA Kit; IMP-3 ELISA Kit; Insulin like growth factor 2 mRNA binding protein 3 ELISA Kit; Insulin-like growth factor 2 mRNA-binding protein 3 ELISA Kit; KH domain containing protein overexpressed in cancer ELISA Kit; KH domain-containing protein overexpressed in cancer ELISA Kit; KOC 1 ELISA Kit; KOC1 ELISA Kit; VICKZ 3 ELISA Kit; VICKZ family member 3 ELISA Kit; VICKZ3 ELISA Kit

  • 缩写：IGF2BP3

  • Uniprot No.：O00425

  • 种属：Homo sapiens (Human)

  • 样本类型：serum, plasma, tissue homogenates, cell lysates

  • 检测范围：37.5 pg/mL-2400 pg/mL

  • 灵敏度：9.37 pg/mL

  • 反应时间：1-5h

  • 样本体积：50-100ul

  • 检测波长：450 nm

  • 研究领域：Epigenetics and Nuclear Signaling

  • 测定原理：quantitative

  • 测定方法：Sandwich

  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%


    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%


    Three samples of known concentration were tested in twenty assays to assess.








  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human IGF2BP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    		SampleSerum(n=4)
    1:1Average %91
    Range %86-95
    1:2Average %102
    Range %97-107
    1:4Average %91
    Range %85-97
    1:8Average %97
    Range %91-103

  • 回收率：

    The recovery of human IGF2BP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5)9288-96
    EDTA plasma (n=4)9793-102














  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected24002.7842.7992.7922.64012001.9561.9061.9311.7796001.0951.1231.1090.9573000.6370.6650.6510.4991500.4190.4510.4350.283750.3380.3420.3400.18837.50.2470.2680.2580.10600.1510.1530.152






  • 货期：3-5 working days

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