首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠胰岛素样生长因子结合蛋白2(IGFBP-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IGFBP2含量。IGFBP2即胰岛素样生长因子结合蛋白2，在多种肿瘤组织中高表达，可调节细胞增殖、存活与迁移等。其研究机制主要聚焦于影响肿瘤微环境、参与信号通路传导，和肿瘤的发生、发展及转移密切相关，是肿瘤治疗潜在的重要靶点。试剂盒检测范围为0.45 ng/mL-30 ng/mL，适用于科研领域对小鼠IGFBP - 2蛋白水平的精准分析，如代谢性疾病模型研究、癌症发生机制探索或生长发育相关实验中的蛋白表达动态监测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Igfbp2 ELISA Kit; Igfbp-2Insulin-like growth factor-binding protein 2 ELISA Kit; IBP-2 ELISA Kit; IGF-binding protein 2 ELISA Kit; IGFBP-2 ELISA Kit; mIGFBP-2 ELISA Kit
缩写：IGFBP2
Uniprot No.：P47877
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.45 ng/mL-30 ng/mL
灵敏度：0.11 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IGFBP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:20 | Average % | 103 | |||||
| Range % | 98-107 | ||||||
| 1:40 | Average % | 84 | |||||
| Range % | 80-88 | ||||||
| 1:80 | Average % | 96 | |||||
| Range % | 92-100 | ||||||
| 1:160 | Average % | 90 | |||||
| Range % | 86-94 | ||||||
回收率：
| The recovery of mouse IGFBP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 88 | 84-92 | |||||
| EDTA plasma (n=4) | 95 | 90-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected 302.6412.4962.5692.470 151.7501.6251.6881.589 7.51.0041.0801.0420.943 3.750.6380.6590.6490.550 1.80.3290.3130.3210.222 0.90.2150.2040.2100.111 0.450.1620.1710.1670.068 00.0960.1020.099 | |||||||
货期：3-5 working days
×
