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Mouse Insulin-like growth factor binding protein 4,IGFBP-4 ELISA kit

  • 中文名： 小鼠胰岛素样生长因子结合蛋白4(IGFBP-4)ELISA Kit
货号: IPD-E07355m
Price： ￥2500
数量：
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  • 产品描述：小鼠胰岛素样生长因子结合蛋白4(IGFBP-4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的IGFBP4含量。IGFBP4是一种胰岛素样生长因子结合蛋白。它在细胞生长、增殖、分化等过程中发挥重要作用。研究发现其能与胰岛素样生长因子结合，调节其活性和功能，影响肿瘤细胞生长、侵袭等。目前在肿瘤等疾病的发生发展机制及治疗研究上受关注。试剂盒检测范围为0.78 ng/mL-50 ng/mL，本试剂盒适用于科研场景中动物模型或体外实验的样本分析，例如探究代谢性疾病机制、生长发育调控或内分泌相关研究，能够为小鼠血清或血浆样本的IGFBP-4动态变化提供可靠数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。

  • 别名：Igfbp4 ELISA Kit; Igfbp-4 ELISA Kit; Insulin-like growth factor-binding protein 4 ELISA Kit; IBP-4 ELISA Kit; IGF-binding protein 4 ELISA Kit; IGFBP-4 ELISA Kit

  • 缩写：IGFBP4

  • Uniprot No.：P47879

  • 种属：Mus musculus (Mouse)

  • 样本类型：serum, plasma

  • 检测范围：0.78 ng/mL-50 ng/mL

  • 灵敏度：0.195 ng/mL

  • 反应时间：1-5h

  • 样本体积：50-100ul

  • 检测波长：450 nm

  • 研究领域：Signal Transduction

  • 测定原理：quantitative

  • 测定方法：Sandwich

  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%



    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%



    Three samples of known concentration were tested in twenty assays to assess.










  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse IGFBP-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    		SampleSerum(n=4)

    1:1Average %92

    Range %88-96

    1:2Average %84

    Range %80-88

    1:4Average %97

    Range %92-101

    1:8Average %105

    Range %100-112

  • 回收率：

    The recovery of mouse IGFBP-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample TypeAverage % RecoveryRange

    Serum (n=5)9590-100

    EDTA plasma (n=4)8884-92

















  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/mlOD1OD2AverageCorrected  502.3842.4952.4402.290  252.0412.1032.0721.922  12.51.6051.5691.5871.437  6.251.0020.9810.9920.842  3.120.5190.5380.5290.379  1.560.3390.3520.3460.196  0.780.2630.2740.2690.119  00.1520.1470.150　  







  • 货期：3-5 working days

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