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产品描述：山羊免疫球蛋白M(IgM)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的IgM含量。IgM是一种免疫球蛋白，是机体初次体液免疫应答中最早产生的抗体。在人体免疫系统中发挥着重要作用，能识别并结合病原体。研究机制上，主要围绕其与抗原的结合特性、激活补体系统等方面展开，以探索其在疾病诊断与治疗中的应用。试剂盒检测范围为24.4 ng/mL-6250 ng/mL，评估疫苗接种后的抗体应答水平、追踪感染性疾病进程中的免疫动态，或探究营养干预对免疫功能的调控机制，为畜牧科研、兽医学研究及动物模型构建提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IgM
种属：Capra hircus (Goat)
样本类型：serum, plasma
检测范围：24.4 ng/mL-6250 ng/mL
灵敏度：24.4 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of goat IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:5000 | Average % | 94 | |||||
| Range % | 89-103 | ||||||
| 1:10000 | Average % | 89 | |||||
| Range % | 81-99 | ||||||
| 1:20000 | Average % | 97 | |||||
| Range % | 89-105 | ||||||
| 1:40000 | Average % | 95 | |||||
| Range % | 83-98 | ||||||
回收率：
| The recovery of goat IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 82-105 | |||||
| EDTA plasma (n=4) | 95 | 88-104 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2Average 62500.3790.3830.381 1562.50.7360.7420.739 390.61.5221.5081.515 97.62.2462.2192.233 24.42.5482.5692.559 02.7562.7832.77 | |||||||
货期：3-5 working days
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