Recombinant Human DIABLO protein

中文名： Diablo同源物(DIABLO)重组蛋白
别名： DIABLO;SMAC;Diablo IAP-binding mitochondrial protein

货号: PA1000-2951

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>85%SDS-PAGE.
种属	Human
靶点	DIABLO
Uniprot No	Q9NR28
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	56-239aa
氨基酸序列	AVPIAQKSEPHSLSSEALMRRAVSLVTDSTSTFLSQTTYALIEAITEYTKAVYTLTSLYRQYTSLLGKMNSEEEDEVWQVIIGARAEMTSKHQEYLKLETTWMTAVGLSEMAAEAAYQTGADQASITARNHIQLVKLQVEEVHQLSRKAETKLAEAQIEELRQKTQEEGEERAESEQEAYLRED
预测分子量	47.4 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于DIABLO(Smac)重组蛋白的3篇经典参考文献，涵盖其功能、结构及重组表达研究：

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1. **文献名称**: *Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition*

**作者**: Du, C., Fang, M., Li, Y., Li, L., & Wang, X.

**摘要**: 该研究首次报道了Smac(即DIABLO)作为线粒体来源的促凋亡蛋白，通过结合并拮抗凋亡抑制蛋白(IAPs)如XIAP，促进caspase-9和caspase-3的活化。研究利用重组Smac蛋白证实其与XIAP-BIR结构域的相互作用，揭示了其通过N端四肽(AVPI)介导的凋亡调控机制。

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2. **文献名称**: *Structural basis of IAP recognition by Smac/DIABLO*

**作者**: Chai, J., Shiozaki, E., Srinivasula, S.M., Wu, Q., Datta, P., Alnemri, E.S., & Shi, Y.

**摘要**: 本研究通过X射线晶体学解析了重组Smac/DIABLO蛋白与XIAP-BIR2结构域的复合物结构，揭示了Smac以二聚体形式通过N端AVPI基序与BIR结构域结合，从而解除XIAP对caspase的抑制。该工作为基于结构的抗癌药物设计提供了理论基础。

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3. **文献名称**: *Engineering a dimeric caspase-9: A re-evaluation of the induced proximity model*

**作者**: Srinivasula, S.M., Datta, P., Fan, X.J., Fernandes-Alnemri, T., Huang, Z., & Alnemri, E.S.

**摘要**: 研究利用重组DIABLO蛋白探讨其在caspase-9激活中的作用，证明DIABLO通过拮抗XIAP促进caspase-9二聚化及活化，并验证了“诱导邻近模型”在凋亡信号中的分子机制。

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**备注**：以上文献发表于2000-2003年，为DIABLO/Smac功能与结构研究的奠基性工作。如需近年研究(如重组DIABLO在肿瘤治疗中的应用)，建议补充关键词(如“DIABLO recombinant protein cancer therapy”)进一步检索。

背景信息

**Background of DIABLO Recombinant Protein**

DIABLO (Direct IAP-Binding Protein with Low pI), also known as SMAC (Second Mitochondrial-derived Activator of Caspases), is a mitochondrial protein critical in regulating apoptosis. It is released into the cytosol during apoptosis in response to cellular stress, where it counteracts Inhibitor of Apoptosis Proteins (IAPs) such as XIAP, cIAP1. and cIAP2. IAPs suppress apoptosis by binding to and inhibiting caspases, the proteases that execute cell death. DIABLO neutralizes this inhibition by physically interacting with IAPs via its N-terminal AVPI motif, displacing caspases and restoring their activity to drive apoptosis.

Structurally, DIABLO contains a mitochondrial targeting sequence (MTS) that directs its localization to the mitochondrial intermembrane space. Upon apoptotic signaling, proteolytic processing removes the MTS, releasing mature DIABLO into the cytosol. Recombinant DIABLO protein is typically produced in bacterial or mammalian expression systems, engineered to exclude the MTS for proper solubility and functionality. Purification methods, such as affinity chromatography, ensure high purity and bioactivity.

Research applications of recombinant DIABLO include studying apoptosis mechanisms, screening small-molecule IAP antagonists for cancer therapy, and structural analyses to elucidate IAP-binding interactions. Its therapeutic potential lies in sensitizing cancer cells to apoptosis, particularly in malignancies resistant to conventional treatments due to elevated IAP levels. However, challenges remain in achieving tumor-specific targeting to avoid off-target toxicity.

Overall, DIABLO recombinant protein serves as a vital tool for both basic research and translational studies aiming to exploit apoptotic pathways for disease treatment.