首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠印度刺猬因子(IHH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IHH含量。小鼠印度刺猬因子(IHH)是一种在胚胎发育过程中起关键作用的基因，其缺失会导致软骨营养不良和发育异常，是引起鸡匍匐性状的突变原因。通过CRISPR/Cas9技术构建的IHH基因敲除细胞系，可用于研究IHH基因功能及其对骨骼发育的影响。试剂盒检测范围为3.12 pg/mL-200 pg/mL，科研人员可通过本产品定量分析小鼠模型中的IHH表达水平，用于探究其在组织再生、骨骼发育异常或相关信号通路调控中的分子机制，也可应用于基因编辑模型验证或疾病病理研究中生物标志物的动态监测，为发育生物学、分子病理学等基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IhhIndian hedgehog protein ELISA Kit; IHH ELISA Kit; HHG-2) [Cleaved into: Indian hedgehog protein N-product; Indian hedgehog protein C-product] ELISA Kit
缩写：IHH
Uniprot No.：P97812
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Stem Cells
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IHH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 86 | |||||
| Range % | 82-91 | ||||||
| 1:2 | Average % | 101 | |||||
| Range % | 98-105 | ||||||
| 1:4 | Average % | 87 | |||||
| Range % | 84-91 | ||||||
| 1:8 | Average % | 100 | |||||
| Range % | 95-105 | ||||||
回收率：
| The recovery of mouse IHH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 89 | 85-94 | |||||
| EDTA plasma (n=4) | 107 | 103-110 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected2001.7851.8311.8081.6871001.1811.2011.1911.070500.7370.8000.7690.648250.4550.4350.4450.32412.50.3010.3120.3070.1866.250.2080.2040.2060.0853.120.1670.1610.1640.04300.1220.1200.121 | |||||||
货期：3-5 working days
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