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产品描述：牛白介素1(IL-1)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL1A含量。IL1A是重要靶点。它属白细胞介素-1家族，在免疫和炎症反应中起关键作用。研究机制方面，聚焦于其信号通路，了解它如何与受体结合、激活下游因子从而引发炎性反应，以此探寻针对性干预手段，为治疗相关疾病提供方向。试剂盒检测范围为250 pg/mL-4000 pg/mL，适用于畜牧科研中免疫机制探索、疫苗效力评估或炎症模型构建等研究场景，为牛类动物免疫相关研究提供标准化工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL1A ELISA Kit; Interleukin-1 alpha ELISA Kit; IL-1 alpha ELISA Kit
缩写：IL-1
Uniprot No.：P08831
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates
检测范围：250 pg/mL-4000 pg/mL
灵敏度：250 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 87-97 | |||||
| 1:4 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 90-102 | |||||
回收率：
| The recovery of bovine IL-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 89 | 83-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 40000.1470.1410.144 20000.2960.2790.288 10000.4310.4670.449 5000.7820.8230.803 2501.8621.8261.844 | ||||||
货期：3-5 working days
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