首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒
产品描述：牛白介素10，IL-10酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL10含量。IL10，即白细胞介素-10，是一种具有抗炎和免疫调节功能的细胞因子。它主要由抗原递呈细胞如活化的T细胞、单核细胞、B细胞和巨噬细胞分泌。IL10通过与其受体结合，激活JAK1和TYK2激酶，进而调节STAT1和STAT3信号通路，抑制Th1细胞因子产生，并抑制抗原呈递细胞的细胞因子产生能力，发挥其抗炎作用。试剂盒检测范围为5 pg/mL-1000 pg/mL，适用于实验室研究牛类炎症相关疾病机制、疫苗免疫效果评估或畜牧领域健康监测等科研用途本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL10Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit
缩写：IL10
Uniprot No.：P43480
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates
检测范围：5 pg/mL-1000 pg/mL
灵敏度：2.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-98 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 87-102 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-104 | |||||
回收率：
| The recovery of bovine IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 95-100 | ||||
| EDTA plasma (n=4) | 96 | 92-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 10000.0980.0950.097 2500.3010.2850.293 800.4870.4580.473 200.9720.9750.974 51.3641.3161.340 02.1282.2112.170 | ||||||
货期：3-5 working days
×
