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产品描述：鸡白介素10(IL-10)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL10含量。IL10是重要的细胞因子，具有抗炎特性，可抑制促炎细胞因子产生。其在维持机体免疫平衡中有关键作用。研究机制上，主要通过与受体结合，激活相关信号通路，抑制炎症介质释放，在炎症性疾病等研究中受广泛关注。试剂盒检测范围为1 pg/mL-200 pg/mL，支持科研人员高效检测鸡感染性疾病模型、疫苗免疫效果评估或免疫调节机制研究中的IL - 10动态变化。适用于家禽免疫学、病理学及生物制剂开发等科研领域本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL10Interleukin-10 ELISA Kit; IL-10 ELISA Kit
缩写：IL10
Uniprot No.：Q6A2H4
种属：Gallus gallus (Chicken)
样本类型：serum, plasma, tissue homogenates
检测范围：1 pg/mL-200 pg/mL
灵敏度：0.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 90-101 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 94-105 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 89-100 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 87-96 | |||||
回收率：
| The recovery of chicken IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 97 | 93-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 2000.1550.1520.154 500.3340.3480.341 160.5150.5020.509 40.9690.9750.972 11.4361.4631.450 02.6582.6672.663 | ||||||
货期：3-5 working days
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