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Mouse interleukin 10,IL-10 ELISA KIT

  • 中文名： 小鼠白介素10(IL-10)ELISA KIT
货号: IPD-E04594m
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    小鼠白介素10(IL-10)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL10含量。IL-10是一种具有多种生物学功能的细胞因子。它作为重要免疫调节因子，能抑制炎症反应和免疫细胞活性。研究机制上，其与受体结合激活信号通路，调控基因表达，在炎症性疾病、自身免疫病等疾病研究中是潜在治疗靶点。试剂盒检测范围为62.5 pg/ml- 4000 pg/ml，灵敏度为15.6 pg/ml。适用于免疫学基础研究，可应用于炎症性疾病动物模型评估、自身免疫机制解析或感染性疾病进程中IL - 10的动态监测，尤其适合需要高灵敏度检测多种生物样本的研究场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Il10 ELISA Kit; Il-10Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit
  • 缩写：
    IL10
  • Uniprot No.：
    P18893
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    62.5 pg/ml - 4000 pg/ml
  • 灵敏度：
    15.6 pg/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    94


    Range %

    85-98


    1:2

    Average %

    97


    Range %

    91-105


    1:4

    Average %

    87


    Range %

    82-98


    1:8

    Average %

    93


    Range %

    86-98


  • 回收率：

    The recovery of mouse IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    100

    94-105


    EDTA plasma (n=4)

    102

    98-109


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    4000

    3.040

    2.903

    2.972

    2.800


    2000

    2.282

    2.167

    2.224

    2.053


    1000

    1.580

    1.628

    1.604

    1.432


    500

    0.919

    0.963

    0.941

    0.770


    250

    0.593

    0.632

    0.613

    0.441


    125

    0.356

    0.365

    0.361

    0.189


    62.5

    0.259

    0.240

    0.250

    0.078


    0

    0.171

    0.173

    0.172



  • 本试剂盒所含材料：



    • A micro ELISA plate --- The 96-well plate has been pre-coated with anti-mouse IL-10 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.

    • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.

    • Biotin-labeled IL-10 antibody (100 x concentrate) 1 x 120 μl ---Act as the detection antibody.

    • HRP-avidin (100 x concentrate) 1 x 120 μl --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.

    • Biotin-antibody Diluent 1 x 15 ml ---Dilute the high concentration Biotin-antibody to an appropriate working solution.

    • HRP-avidin Diluent 1 x 15 ml ---Dilute the high concentration HRP-avidin solution to an appropriate solution.

    • 本试剂盒不含材料：



      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

      • An incubator can provide stable incubation conditions up to 37°C±5°C.

      • Centrifuge

      • Vortex

      • Squirt bottle, manifold dispenser, or automated microplate washer

      • Absorbent paper for blotting the microtiter plate


      • 货期：
        3-5 working days

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