首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒
产品描述：猪白介素10(IL-10)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL10含量。IL10是一种重要的细胞因子。其背景在于具有免疫调节功能，可抑制炎症反应。研究机制方面，它主要通过与受体结合，激活细胞内信号通路，调节免疫细胞的活性和功能，减少促炎因子产生，在免疫相关疾病研究中备受关注。试剂盒检测范围为6.25 pg/mL-400 pg/mL，适用于动物免疫机制研究、疫苗效力评估或炎症性疾病模型的生物标志物分析。该产品为兽医病理学、比较医学及畜牧养殖领域的免疫调控研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL10Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit
缩写：IL10
Uniprot No.：Q29055
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 81-103 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 90-105 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-108 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 82-112 | |||||
回收率：
| The recovery of pig IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 85-109 | ||||
| EDTA plasma (n=4) | 97 | 92-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected4002.1782.1522.1652.0152001.5841.5601.5721.4221000.9810.9500.9660.816500.5680.5640.5660.416250.3720.3610.3670.21712.50.2960.3020.2990.1496.250.2520.2480.2500.10000.1520.1480.150 | ||||||
货期：3-5 working days
×
