首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔子白介素10(IL-10)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL10含量。IL10是一种重要的免疫调节细胞因子。它可抑制多种促炎细胞因子的产生，从而发挥抗炎作用。目前关于IL10的研究聚焦于其在自身免疫病、感染性疾病等疾病中的作用机制，有望为相关疾病的治疗提供新靶点和新思路。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，该产品广泛应用于免疫学研究领域，特别适用于评估炎症性疾病模型、感染免疫应答或免疫调节药物干预实验中IL-10的动态变化，为探索免疫调控机制提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL10 ELISA Kit; Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit
缩写：IL10
Uniprot No.：Q9TSJ4
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 80-98 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-110 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-100 | |||||
回收率：
| The recovery of rabbit IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 89 | 80-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.6272.5492.5882.46110001.9541.7991.8771.7505001.0040.9870.9960.8692500.5990.5540.5770.4501250.3690.3610.3650.23862.50.2740.2640.2690.14231.250.1770.1710.1740.04700.1320.1220.127 | ||||||
货期：3-5 working days
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