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产品描述：人白介素11(IL-11)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、组织培养上清液、尿液、组织匀浆样本中的IL11含量。IL11是一种促炎性细胞因子，属于IL-6家族，参与多种生理过程，如造血、骨骼发育和免疫反应。随着年龄增长，IL11水平上升，与衰老相关病理变化有关。研究发现，IL11通过调节ERK-AMPK-mTORC1信号通路影响细胞和组织衰老，抑制IL11可延长寿命。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于体外研究IL-11在细胞信号传导、组织修复、肿瘤微环境调控等领域的表达动态，为炎症性疾病模型构建、药物干预效果评估及分子机制探索提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Adipogenesis inhibitory factor ELISA Kit; AGIF ELISA Kit; IL 11 ELISA Kit; IL-11 ELISA Kit; Il11 ELISA Kit; IL11_HUMAN ELISA Kit; Interleukin 11 ELISA Kit; Interleukin-11 ELISA Kit; Oprelvekin ELISA Kit
缩写：IL11
Uniprot No.：P20809
种属：Homo sapiens (Human)
样本类型：serum, cell culture supernates, urine, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL-11 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 94-102 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 84-93 | |||||
回收率：
| The recovery of human IL-11 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 84 | 80-88 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.8382.7312.7852.66510002.1402.1022.1212.0015001.4161.3261.3711.2512500.9030.8670.8850.7651250.5020.5160.5090.38962.50.3300.3270.3290.20931.250.2640.2530.2590.13900.1190.1210.120 | ||||||
货期：3-5 working days
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