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产品描述：山羊白介素10(IL-10)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL10含量。IL10，又称白细胞介素-10，是一种多效性细胞因子，主要由单核细胞和T细胞产生。它具有抗炎和免疫调节功能，通过抑制Th1细胞因子和抗原呈递细胞的功能来发挥其作用。IL10的研究机制涉及信号转导和转录激活因子STAT1、STAT3的激活，以及与其他细胞因子的相互作用。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于科研领域研究山羊模型中的免疫应答机制、炎症性疾病进展评估或疫苗开发中的免疫调节分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IL-10
Uniprot No.：A7Y3X5
种属：Capra hircus (Goat)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of goat IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 82 | ||||
| Range % | 80-85 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 84-93 | |||||
| 1:8 | Average % | 107 | ||||
| Range % | 103-111 | |||||
回收率：
| The recovery of goat IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 85 | 80-90 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.7252.7682.7472.61510002.0592.0002.0301.8985001.3211.3681.3451.2132500.7410.7580.7500.6181250.4550.4320.4440.31262.50.3070.3010.3040.17231.250.2280.2260.2270.09500.1310.1330.132 | ||||||
货期：3-5 working days
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