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Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. |
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-11 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 88 | ||||
Range % | 80-92 | |||||
1:2 | Average % | 92 | ||||
Range % | 88-96 | |||||
1:4 | Average % | 95 | ||||
Range % | 91-105 | |||||
1:8 | Average % | 94 | ||||
Range % | 86-98 |
The recovery of mouse IL-11 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 95 | 89-102 | ||||
EDTA plasma (n=4) | 93 | 90-99 |
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
pg/ml | OD1 | OD2 | Average | Corrected | ||
5000 | 2.597 | 2.654 | 2.626 | 2.478 | ||
2500 | 1.733 | 1.667 | 1.700 | 1.552 | ||
1250 | 1.163 | 1.098 | 1.130 | 0.982 | ||
625 | 0.740 | 0.729 | 0.734 | 0.586 | ||
312 | 0.434 | 0.421 | 0.428 | 0.279 | ||
156 | 0.325 | 0.343 | 0.334 | 0.186 | ||
78 | 0.219 | 0.204 | 0.211 | 0.063 | ||
0 | 0.151 | 0.145 | 0.148 |
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