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Mouse Interleukin 11,IL-11 ELISA KIT

  • 中文名: 小鼠白介素11(IL-11)ELISA Kit
货号: IPD-E04597m
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    小鼠白介素11(IL-11)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的IL11含量。IL11,即白细胞介素-11,是一种由多种细胞类型产生的多功能细胞因子,主要作用是刺激骨髓造血干细胞的增殖、成熟和形成集落。它还能促进巨核细胞的形成和成熟,提高血小板数量,并参与免疫调节。IL11的研究涉及其基因结构、生物学功能、信号传导途径以及在衰老和疾病中的作用。试剂盒检测范围为78 pg/ml- 5000 pg/ml,灵敏度为19.5 pg/ml。该产品适用于炎症性疾病模型评估、免疫细胞功能研究、肿瘤微环境分析等基础科研领域本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    Il11Interleukin-11 ELISA Kit; IL-11 ELISA Kit
  • 缩写:
    IL11
  • Uniprot No.:
    P47873
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    78 pg/ml - 5000 pg/ml
  • 灵敏度:
    19.5 pg/ml
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-11 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    88


    Range %

    80-92


    1:2

    Average %

    92


    Range %

    88-96


    1:4

    Average %

    95


    Range %

    91-105


    1:8

    Average %

    94


    Range %

    86-98


  • 回收率:

    The recovery of mouse IL-11 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    95

    89-102


    EDTA plasma (n=4)

    93

    90-99


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    5000

    2.597

    2.654

    2.626

    2.478


    2500

    1.733

    1.667

    1.700

    1.552


    1250

    1.163

    1.098

    1.130

    0.982


    625

    0.740

    0.729

    0.734

    0.586


    312

    0.434

    0.421

    0.428

    0.279


    156

    0.325

    0.343

    0.334

    0.186


    78

    0.219

    0.204

    0.211

    0.063


    0

    0.151

    0.145

    0.148




  • 货期:
    3-5 working days

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