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产品描述：牛白介素12(IL-12)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL12A含量。IL12A为重要靶点，在免疫系统中扮演关键角色。其背景上，IL-12由IL12A等亚基组成，可调节免疫反应。研究机制方面，它能促进T细胞和NK细胞的活化、增殖，增强免疫细胞对病原体和肿瘤细胞的杀伤作用，是免疫治疗的研究热点。试剂盒检测范围为6.25 pg/mL-400 pg/mL，该产品为研究牛类感染性疾病、炎症反应机制及疫苗免疫效果评估提供可靠工具，尤其适用于畜牧科研中免疫调节机制探索、疾病模型建立等基础研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL12AInterleukin-12 subunit alpha ELISA Kit; IL-12A ELISA Kit; Cytotoxic lymphocyte maturation factor 35 kDa subunit ELISA Kit; CLMF p35 ELISA Kit; IL-12 subunit p35 ELISA Kit
缩写：IL12A
Uniprot No.：P54349
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 91-102 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 86-99 | |||||
回收率：
| The recovery of bovine IL-12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 84-92 | ||||
| EDTA plasma (n=4) | 97 | 95-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected4002.7582.6282.6932.5312002.1842.0842.1341.9721001.6121.5621.5871.425501.2031.1731.1881.026250.6520.6420.6470.48512.50.3840.3740.3790.2176.250.2580.2530.2560.09400.1630.1610.162 | ||||||
货期：3-5 working days
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