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产品描述：猪白介素12(IL-12/P40)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的IL12B含量。IL12B是一种编码白细胞介素12亚单位的基因，参与T细胞和自然杀伤细胞的免疫反应。其研究机制涉及IL12B与IL12A结合形成异源二聚体，激活Jak-STAT信号通路，调节免疫应答和炎症反应。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，其水平检测可为感染性疾病、自身免疫研究及疫苗开发等科研领域提供重要参考；适用于评估实验动物模型中的免疫应答强度、细胞因子网络调控机制研究，或体外药物筛选过程中炎症介质的动态监测；为科研人员探索猪源IL - 12/P40在免疫病理学中的功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL12BInterleukin-12 subunit beta ELISA Kit; IL-12B ELISA Kit; Cytotoxic lymphocyte maturation factor 40 kDa subunit ELISA Kit; CLMF p40 ELISA Kit; IL-12 subunit p40 ELISA Kit
缩写：IL12B
Uniprot No.：Q28938
种属：Sus scrofa (Pig)
样本类型：serum, plasma, cell culture supernates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-12/P40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 80-99 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 90-104 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 92-110 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 85-105 | |||||
回收率：
| The recovery of pig IL-12/P40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 85-104 | ||||
| EDTA plasma (n=4) | 98 | 90-112 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.0282.1572.0931.8845001.5621.6121.5871.3782500.9801.0341.0070.7981250.5700.6030.5870.37862.50.3520.3620.3570.14831.250.3030.3110.3070.09815.60.2820.2680.2750.06600.2140.2040.209 | ||||||
货期：3-5 working days
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