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产品描述：犬白介素13(IL13)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL13含量。IL13 是重要的细胞因子，在免疫调节等方面有重要作用。其异常表达与哮喘、特应性皮炎等多种疾病相关。研究机制主要聚焦于其与受体结合后激活下游信号通路，从而参与炎症反应、细胞增殖分化等过程，是治疗相关疾病的潜在靶点。试剂盒检测范围为12.5 pg/mL-800 pg/mL，适用于科研领域对犬类免疫调节机制的研究。科研人员可将其应用于犬类过敏性疾病模型构建、寄生虫感染后免疫应答评估、药物干预效果分析等研究，为探索犬类Th2型免疫反应提供可靠的工具支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL13 ELISA Kit; Interleukin-13 ELISA Kit; IL-13 ELISA Kit
缩写：IL13
Uniprot No.：Q9N0W9
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, tissue homogenates
检测范围：12.5 pg/mL-800 pg/mL
灵敏度：3.12 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of dog IL13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 98 | |||||
| Range % | 93-102 | ||||||
| 1:2 | Average % | 92 | |||||
| Range % | 88-96 | ||||||
| 1:4 | Average % | 90 | |||||
| Range % | 86-94 | ||||||
| 1:8 | Average % | 87 | |||||
| Range % | 82-91 | ||||||
回收率：
| The recovery of dog IL13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 104 | 100-112 | |||||
| EDTA plasma (n=4) | 85 | 80-90 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected8002.3152.4672.3912.2634002.0492.1582.1041.9762001.7201.6801.7001.5721001.1931.1241.1591.031500.7820.7960.7890.661250.5070.4750.4910.36312.50.3280.3090.3190.19100.1240.1310.128 | |||||||
货期：3-5 working days
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