Mouse Interleukin 13,IL-13 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E04602m

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠白介素13(IL-13)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL13含量。IL13是一种免疫调节细胞因子，主要由活化的Th2细胞产生，参与过敏性炎症的调节，对淋巴细胞、骨髓细胞和非造血细胞的反应有重要作用。其在癌症、哮喘和过敏性疾病的发病机制和治疗中扮演关键角色。研究显示，IL13通过不依赖STAT6的途径促进肿瘤侵袭、转移和TGFβ的产生。试剂盒检测范围为78 pg/ml- 5000 pg/ml，灵敏度为19.5 pg/ml。适用于体外实验中对小鼠疾病模型中IL - 13表达水平的动态监测、药物干预效果评估或免疫调控机制研究，为炎症相关科研提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Il13 ELISA Kit; Il-13 ELISA Kit; Interleukin-13 ELISA Kit; IL-13 ELISA Kit; T-cell activation protein P600 ELISA Kit
缩写：
IL13
Uniprot No.：
P20109
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates
检测范围：
78 pg/ml - 5000 pg/ml
灵敏度：
19.5 pg/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-98
1:2	Average %	95
	Range %	91-105
1:4	Average %	94
	Range %	90-105
1:8	Average %	93
	Range %	86-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
5000	3.010	2.934	2.972	2.812
2500	2.168	2.267	2.218	2.057
1250	1.290	1.328	1.309	1.149
625	0.773	0.769	0.771	0.611
312	0.448	0.421	0.435	0.275
156	0.324	0.343	0.333	0.173
78	0.243	0.234	0.239	0.079
0	0.165	0.155	0.160