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产品描述:豚鼠白介素13(IL-13)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的IL13含量。IL13是一种免疫调节细胞因子,参与过敏性炎症和癌症的发病机制。主要由活化的TH2细胞分泌,与多种细胞相互作用,调节免疫反应。研究IL13有助于了解相关疾病的发生和发展,为疾病治疗提供新靶点。试剂盒检测范围为7.5 pg/mL-300 pg/mL,适用于豚鼠感染模型、呼吸道炎症研究、免疫调节药物开发等科研场景,能够稳定检测包括肺组织匀浆在内的多种生物样本,为探索IL-13在免疫调控网络中的作用及评估实验性治疗方案的生物学效应提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
缩写:IL-13
Uniprot No.:H0W834
种属:Guinea Sus scrofa (Pig)
样本类型:serum, plasma, tissue homogenates
检测范围:7.5 pg/mL-300 pg/mL
灵敏度:3.75 pg/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Immunology
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<15% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<15% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of Guinea pig IL-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1 | Average % | 88 | |||||
Range % | 83-92 | ||||||
1:2 | Average % | 95 | |||||
Range % | 85-105 | ||||||
1:4 | Average % | 93 | |||||
Range % | 86-102 | ||||||
1:8 | Average % | 97 | |||||
Range % | 92-110 |
回收率:
The recovery of Guinea pig IL-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 96 | 91-103 | |||||
EDTA plasma (n=4) | 93 | 86-99 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
pg/mlOD1OD2Average3002.2442.2672.2561501.1731.1611.167750.6280.6500.63937.50.3590.3430.351150.1520.1510.1527.50.0830.0790.081 |
货期:3-5 working days
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