Human Interleukin 15,IL-15 ELISA KIT

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E04603h

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
人白介素15(IL-15)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL15含量。IL15是重要细胞因子，在免疫调节等生理过程中起关键作用。其研究机制涉及调控免疫细胞，如激活NK细胞和记忆性CD8⁺T细胞，增强机体免疫功能，在肿瘤免疫治疗、自身免疫病研究等领域受关注，有望带来新治疗策略。试剂盒检测范围为3.12 pg/ml- 200 pg/ml，灵敏度为0.78 pg/ml。适用于体外培养模型、动物实验或人体样本的IL - 15表达水平研究；为免疫调控机制探索、疾病模型构建或药物干预效果评估等科研场景提供高效解决方案；满足实验室对复杂生物样本中微量蛋白的定量需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
IL 15 ELISA Kit; IL-15 ELISA Kit; IL15 ELISA Kit; IL15_HUMAN ELISA Kit; Interleukin 15 ELISA Kit; Interleukin-15 ELISA Kit; Interleukin15 ELISA Kit; MGC9721 ELISA Kit
缩写：
IL15
Uniprot No.：
P40933
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
3.12 pg/ml - 200 pg/ml
灵敏度：
0.78 pg/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human IL-15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-93
1:2	Average %	94
	Range %	91-99
1:4	Average %	90
	Range %	85-96
1:8	Average %	96
	Range %	86-100

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
200	2.536	2.436	2.486	2.345
100	2.001	1.901	1.951	1.810
50	1.308	1.208	1.258	1.117
25	0.815	0.805	0.810	0.669
12.5	0.453	0.443	0.448	0.307
6.25	0.324	0.314	0.319	0.178
3.12	0.231	0.222	0.227	0.086
0	0.141	0.140	0.141