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产品描述：鸡白介素17(IL-17)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL17含量。IL-17是一种由Th17细胞分泌的促炎细胞因子，参与调节免疫反应和炎症过程。其在银屑病、类风湿关节炎等自身免疫疾病中起重要作用，同时也是肿瘤发生和进展的关键因子。研究IL-17机制有助于开发针对相关疾病的治疗方法。试剂盒检测范围为150 pg/mL-6000 pg/mL，适用于家禽免疫学研究领域，如探究病原体感染后宿主免疫应答动态、评估疫苗免疫效果或解析炎症性疾病模型中的细胞因子调控网络，为禽类基础免疫机制研究及疾病防控提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IL-17
Uniprot No.：Q7T1P7
种属：Gallus gallus (Chicken)
样本类型：serum, plasma, tissue homogenates
检测范围：150 pg/mL-6000 pg/mL
灵敏度：150 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 82-100 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 90-101 | |||||
| 1:8 | Average % | 91 | ||||
| Range % | 85-98 | |||||
回收率：
| The recovery of chicken IL-17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 85-96 | ||||
| EDTA plasma (n=4) | 92 | 86-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 01.9142.0271.971 1501.3061.3291.318 4501.0391.1021.071 13800.7550.7020.729 30000.4840.4620.473 60000.3510.3480.350 | ||||||
货期：3-5 working days
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