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产品描述：人白介素15受体α亚基(IL15RA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL15RA含量。IL15RA是一种重要靶点。IL-15与其受体IL15RA结合，在免疫调节等生理过程中发挥关键作用。科研聚焦其与多种免疫细胞相互作用机制，探索通过调控IL15RA信号通路来开发治疗自身免疫病、肿瘤等疾病的新疗法。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于免疫调控机制研究、药物靶点验证、疾病模型构建等科研场景，可支持体外实验中对免疫相关分子网络的动态监测与功能解析，为免疫学基础研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AA690181 ELISA Kit; CD215 ELISA Kit; I15RA_HUMAN ELISA Kit; IL 15R alpha ELISA Kit; IL-15 receptor subunit alpha ELISA Kit; IL-15R-alpha ELISA Kit; IL-15RA ELISA Kit; Il15ra ELISA Kit; Interleukin 15 receptor alpha ELISA Kit; Interleukin 15 receptor subunit alpha ELISA Kit; MGC104179 ELISA Kit; sIL-15 receptor subunit alpha ELISA Kit; sIL-15R-alpha ELISA Kit; sIL-15RA ELISA Kit; Soluble interleukin 15 receptor subunit alpha ELISA Kit; Soluble interleukin-15 receptor subunit alpha ELISA Kit
缩写：IL15RA
Uniprot No.：Q13261
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL15RA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 93 | |||||
| Range % | 88-97 | ||||||
| 1:2 | Average % | 95 | |||||
| Range % | 91-99 | ||||||
| 1:4 | Average % | 92 | |||||
| Range % | 87-97 | ||||||
| 1:8 | Average % | 89 | |||||
| Range % | 85-93 | ||||||
回收率：
| The recovery of human IL15RA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 91 | 88-93 | |||||
| EDTA plasma (n=4) | 104 | 101-108 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected20002.7662.8442.8052.72610002.2432.2532.2482.1695001.3481.3661.3571.2782500.7710.8050.7880.7091250.3520.3660.3590.28062.50.2560.2770.2670.18831.250.1490.1540.1520.07300.0770.0800.079 | |||||||
货期：3-5 working days
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