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产品描述：猪白介素15(IL-15)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、细胞裂解物样本中的IL15含量。IL-15是一种细胞因子，在免疫系统调节中至关重要。它能促进T细胞和NK细胞的存活、增殖及活化，增强机体免疫功能。目前关于它的研究聚焦于其在肿瘤免疫治疗、自身免疫病等方面的机制，为相关疾病提供新的治疗思路。试剂盒检测范围为0.78 pg/mL-50 pg/mL，该产品广泛应用于动物免疫学、疾病机制研究及疫苗开发等领域，例如用于评估猪群免疫状态、研究病原体感染过程中细胞因子的动态变化，或分析体外免疫细胞培养模型中IL - 15的分泌水平，为科研人员探索猪类免疫调控网络提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL15 ELISA Kit; Interleukin-15 ELISA Kit; IL-15 ELISA Kit
缩写：IL15
Uniprot No.：Q95253
种属：Sus scrofa (Pig)
样本类型：serum, plasma, cell culture supernates, tissue homogenates, cell lysates
检测范围：0.78 pg/mL-50 pg/mL
灵敏度：0.195 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 80-89 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:4 | Average % | 87 | ||||
| Range % | 84-91 | |||||
| 1:8 | Average % | 84 | ||||
| Range % | 81-89 | |||||
回收率：
| The recovery of pig IL-15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 97-103 | ||||
| EDTA plasma (n=4) | 84 | 80-88 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected502.6022.5762.5892.400251.8791.8501.8651.67612.51.1441.1681.1560.9676.250.6830.6910.6870.4983.120.4520.4680.4600.2711.560.3610.3910.3760.1870.780.2880.2980.2930.10400.1910.1860.189 | ||||||
货期：3-5 working days
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