Mouse Interleukin 15,IL-15 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E04604m

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠白介素15(IL-15)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL15含量。IL15是重要的细胞因子。其背景在于它参与免疫调节，在机体抗感染、抗肿瘤等免疫反应中有重要作用。研究机制方面，IL15可激活NK细胞和CD8⁺T细胞活性，增强其杀伤功能，还参与调节T细胞增殖、分化，是免疫治疗领域的潜在靶点。试剂盒检测范围为0.3125 - 20ng/ml，灵敏度为0.078 ng/ml。适用于免疫学基础研究、炎症反应机制探索及疾病模型构建等领域，例如在感染性疾病模型、自身免疫性疾病研究或肿瘤微环境分析中，量化 IL - 15 的表达水平变化；支持实验室常规检测需求，满足细胞因子动态监测和生物标志物筛选等科研场景应用本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Il15 ELISA Kit; Interleukin-15 ELISA Kit; IL-15 ELISA Kit
缩写：
IL15
Uniprot No.：
P48346
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates
检测范围：
0.312ng/ml - 20ng/ml
灵敏度：
0.078 ng/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-92
1:2	Average %	90
	Range %	85-96
1:4	Average %	93
	Range %	91-105
1:8	Average %	93
	Range %	86-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
20	2.187	2.054	2.120	1.951
10	1.413	1.467	1.440	1.270
5	0.879	0.928	0.904	0.734
2.5	0.589	0.609	0.599	0.429
1.25	0.355	0.341	0.348	0.178
0.625	0.283	0.273	0.278	0.108
0.312	0.222	0.214	0.218	0.048
0	0.175	0.165	0.170