| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SNAPC1 |
| Uniprot No | Q16533 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-368aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMGTPPGLQTDCEALLSRFQETDSVRFE DFTELWRNMKFGTIFCGRMRNLEKNMFTKEALALAWRYFLPPYTFQIRVG ALYLLYGLYNTQLCQPKQKIRVALKDWDEVLKFQQDLVNAQHFDAAYIFR KLRLDRAFHFTAMPKLLSYRMKKKIHRAEVTEEFKDPSDRVMKLITSDVL EEMLNVHDHYQNMKHVISVDKSKPDKALSLIKDDFFDNIKNIVLEHQQWH KDRKNPSLKSKTNDGEEKMEGNSQETERCERAESLAKIKSKAFSVVIQAS KSRRHRQVKLDSSDSDSASGQGQVKATRKKEKKERLKPAGRKMSLRNKGN VQNIHKEDKPLSLSMPVITEEEENESLSGTEFTASKKRRKH |
| 预测分子量 | 45 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SNAPC1重组蛋白的参考文献示例(内容为示例性虚构，仅作参考)：
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1. **文献名称**：Structural and Functional Analysis of SNAPC1 in snRNA Transcription
**作者**：Smith J, et al.
**摘要**：本研究通过大肠杆菌表达系统成功纯化重组SNAPC1蛋白，结合X射线晶体学解析其三维结构，揭示其N端结构域在结合RNA聚合酶II和启动子识别中的关键作用，为snRNA转录机制提供结构基础。
2. **文献名称**：Recombinant SNAPC1 Interactions with the snRNA Activation Complex
**作者**：Chen L, Wang H
**摘要**：利用昆虫细胞表达系统获得高纯度SNAPC1重组蛋白，通过体外pull-down实验证明其与SNAPC4、PTF1A的直接互作，并证实这些相互作用对U6 snRNA转录起始复合物的组装至关重要。
3. **文献名称**：Role of SNAPC1 in RNA Polymerase III Recruitment: Insights from Recombinant Protein Assays
**作者**：Kimura T, et al.
**摘要**：通过重组表达人源SNAPC1蛋白，结合凝胶迁移实验(EMSA)发现其直接结合U1 snRNA启动子保守序列，并证明其C端结构域缺失会显著降低RNA聚合酶III的招募效率。
4. **文献名称**：Disease-Linked Mutations in SNAPC1 Disrupt snRNA Transcription: Evidence from Recombinant Reconstitution
**作者**：Garcia R, et al.
**摘要**：构建携带临床突变(如R258Q)的重组SNAPC1蛋白，发现其与SNAPC复合物的结合能力受损，导致体外转录活性下降，提示SNAPC1突变可能通过破坏snRNA合成引发相关神经发育疾病。
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注：以上文献信息为模拟示例，实际引用需以真实数据库(如PubMed、Web of Science)检索结果为准。
**Background of SNAPC1 Recombinant Protein**
SNAPC1 (Small Nuclear RNA Activating Complex Polypeptide 1) is a critical subunit of the Small Nuclear RNA (snRNA) Activating Complex (SNAPc), a multi-protein assembly essential for the transcription of snRNA genes by RNA polymerase II (Pol II) and III (Pol III). SnRNAs are non-coding RNAs involved in pre-mRNA splicing, RNA processing, and gene regulation. SNAPc binds to the proximal sequence element (PSE) in snRNA gene promoters, facilitating the recruitment of Pol II or Pol III and enabling transcription initiation.
The SNAPC1 subunit plays a structural and functional role in stabilizing the SNAPc complex and mediating interactions with other transcriptional regulators. Recombinant SNAPC1 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells*) to produce purified, biologically active protein for *in vitro* studies. It typically retains the ability to bind DNA and interact with other SNAPc subunits, making it valuable for investigating snRNA transcription mechanisms, protein-DNA interactions, and dysregulation in diseases like cancers or neurodegenerative disorders.
Research applications include chromatin immunoprecipitation (ChIP), electrophoretic mobility shift assays (EMSA), and structural studies (e.g., X-ray crystallography or cryo-EM) to map DNA-binding domains or resolve SNAPc architecture. Recombinant SNAPC1 also aids in developing therapeutic strategies targeting transcription anomalies. Its production often involves affinity tags (e.g., His-tag) for efficient purification and detection. Studies using this protein have advanced understanding of snRNA biogenesis, Pol II/III specificity, and the role of SNAPc in cellular homeostasis.
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