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产品描述：猪白介素17(IL-17)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL17A含量。IL17A是一种重要的细胞因子。其背景在于它在多种炎症性疾病及自身免疫病病理过程中扮演关键角色。研究机制上，IL17A能调节免疫细胞功能、促进炎性介质分泌等，以此影响疾病发生发展，是当前免疫和炎症领域研究热点。试剂盒检测范围为20 pg/mL- 800 pg/mL，适用于体外实验环境，可稳定检测多种生物样本中IL - 17的表达水平，为动物免疫学、病原微生物感染研究以及抗炎药物开发提供可靠数据支持。IL - 17的动态监测对猪类免疫机制研究、炎症性疾病模型构建及疫苗效果评估具有重要科研价值。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL17A ELISA Kit; IL17 ELISA Kit; Interleukin-17A ELISA Kit; IL-17 ELISA Kit; IL-17A ELISA Kit
缩写：IL17A
Uniprot No.：Q60I29
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：20 pg/mL- 800 pg/mL
灵敏度：10 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 86-96 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 92-106 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 83-94 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 87-98 | |||||
回收率：
| The recovery of pig IL-17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-99 | ||||
| EDTA plasma (n=4) | 84 | 80-88 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 8002.8062.8622.834 4002.0802.0972.089 2001.1741.1981.186 1000.6980.6630.681 400.2810.2940.288 200.1470.1450.146 | ||||||
货期：3-5 working days
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