首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人白介素17F (IL17F)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的IL17F含量。IL17F是重要靶点。它是IL-17细胞因子家族成员，参与机体免疫防御，也与自身免疫病密切相关。研究机制上，主要聚焦于其信号通路，探索阻断其信号传递来治疗银屑病、类风湿关节炎等疾病，为相关药物研发提供方向。试剂盒检测范围为12.5 pg/mL-800 pg/mL，细胞因子信号通路研究、炎症模型建立、药物靶点筛选、免疫学研究、疾病机制探索、生物制剂开发等科研场景，所有应用场景均限于科学研究领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CANDF6 ELISA Kit; Cytokine ML 1 ELISA Kit; Cytokine ML-1 ELISA Kit; IL 17F ELISA Kit; IL 24 ELISA Kit; IL-17F ELISA Kit; IL-24 ELISA Kit; Il17f ELISA Kit; IL17F_HUMAN ELISA Kit; Interleukin 17F ELISA Kit; Interleukin 24 ELISA Kit; Interleukin-17F ELISA Kit; Interleukin-24 ELISA Kit; ML 1 ELISA Kit; ML1 ELISA Kit; Mutant IL 17F ELISA Kit; OTTHUMP00000016602 ELISA Kit
缩写：IL17F
Uniprot No.：Q96PD4
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates
检测范围：12.5 pg/mL-800 pg/mL
灵敏度：3.1 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL17F in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 87-99 | |||||
| 1:2 | Average % | 103 | ||||
| Range % | 99-108 | |||||
| 1:4 | Average % | 80 | ||||
| Range % | 85-96 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 85-98 | |||||
回收率：
| The recovery of human IL17F spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-96 | ||||
| EDTA plasma (n=4) | 89 | 85-93 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected8001.8501.9201.8851.7954001.3421.4051.3741.2842000.8340.9020.8680.7781000.5570.5250.5410.451500.3250.3420.3340.244250.2530.2640.2590.16912.50.1460.1500.1480.05800.0870.0920.090 | ||||||
货期：3-5 working days
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