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产品描述：鸡白介素18(IL-18)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL18含量。IL-18，又称干扰素-γ诱导因子，是一种促炎细胞因子，属于IL-1超家族。它主要由巨噬细胞和其他细胞产生，能够诱导T细胞产生干扰素-γ。IL-18在免疫调节中起重要作用，参与先天性和适应性免疫反应的调节。其研究机制涉及IL-18与IL-18R的结合，以及通过激活下游信号通路来调节免疫细胞的功能。试剂盒检测范围为20 pg/mL-500 pg/mL，适用于家禽免疫学研究、疫苗效力评估或感染性疾病模型的建立。科研人员可通过精准分析鸡类样本中IL - 18的表达水平，探究其在传染性法氏囊病、新城疫等病原体感染过程中的免疫调控机制，或评估营养干预、环境应激对家禽免疫功能的影响。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL18 ELISA Kit; Interleukin-18 ELISA Kit; IL-18 ELISA Kit
缩写：IL18
Uniprot No.：Q8QFQ8
种属：Gallus gallus (Chicken)
样本类型：serum, plasma, tissue Homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 89-102 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 82-99 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 85-102 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 92-100 | |||||
回收率：
| The recovery of chicken IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 81-99 | ||||
| EDTA plasma (n=4) | 95 | 89-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 5001.9811.9271.9541.851 2501.3341.3741.3541.251 1250.9200.9150.9180.815 62.50.6190.6430.6310.528 31.20.3950.3760.3860.283 15.60.2900.3060.2980.195 7.80.2110.2240.2180.115 00.1020.1030.103 | ||||||
货期：3-5 working days
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