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产品描述：兔子白介素18(IL-18)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的IL-18含量。IL-18是一个重要靶点。它属于白细胞介素家族，在免疫调节中发挥关键作用。研究发现，IL-18参与多种炎症相关疾病发生发展。其作用机制主要是通过结合受体激活下游信号通路，调控免疫细胞功能，为相关疾病治疗提供了新方向。试剂盒检测范围为12.5 pg/mL-800 pg/mL，适用于科研场景中IL - 18浓度的精准分析，例如免疫机制研究、炎症动物模型评估或疫苗开发中免疫原性评价。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IL-18
Uniprot No.：B1A3U4
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma
检测范围：12.5 pg/mL-800 pg/mL
灵敏度：3.12 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of rabbit IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 102 | 92-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected8002.9752.8522.9142.8104002.0882.0762.0821.9782001.2051.1671.1861.0821000.5700.5900.5800.476500.3270.3340.3310.227250.2130.2080.2110.10712.50.1450.1400.1430.03900.1050.1030.104 | ||||||
货期：3-5 working days
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