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Mouse interleukin-18 binding prorein(IL-18BP) ELISA Kit

  • 中文名: 小鼠白介素18结合蛋白(IL-18BP)ELISA Kit
货号: IPD-E17797m
Price: ¥2500
数量:
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产品详情

  • 产品描述:小鼠白介素18结合蛋白(IL-18BP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织培养上清液样本中的IL-18BP含量。IL-18BP(白介素-18结合蛋白)是一种免疫检查点蛋白,可抑制IL-18与其受体结合,调节Th1细胞因子反应。其在多种肿瘤和自身免疫性疾病中高表达,研究机制涉及IL-18与IL-18BP的相互作用及其对免疫反应的影响。试剂盒检测范围为0.156 ng/mL-10 ng/mL,科研人员可将其应用于炎症性疾病动物模型的机制研究、药物干预效果评估或体外细胞实验中IL-18/IL-18BP信号通路的动态监测,特别适用于探索类风湿性关节炎、炎症性肠病等病理过程中IL-18通路的调控机制本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。

  • 别名:Il18bp ELISA Kit; Igifbp ELISA Kit; Interleukin-18-binding protein ELISA Kit; IL-18BP ELISA Kit; Interferon gamma-inducing factor-binding protein ELISA Kit

  • 缩写:IL-18BP

  • Uniprot No.:Q9Z0M9

  • 种属:Mus musculus (Mouse)

  • 样本类型:serum, plasma, cell culture supernates

  • 检测范围:0.156 ng/mL-10 ng/mL

  • 灵敏度:0.039 ng/mL

  • 反应时间:1-5h

  • 样本体积:50-100ul

  • 检测波长:450 nm

  • 研究领域:Immunology

  • 测定原理:quantitative

  • 测定方法:Sandwich

  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%


    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%


    Three samples of known concentration were tested in twenty assays to assess.








  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-18BP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    SampleSerum(n=4)
    1:1Average %97
    Range %93-100
    1:2Average %84
    Range %80-88
    1:4Average %104
    Range %100-107
    1:8Average %91
    Range %87-95
  • 回收率:

    The recovery of mouse IL-18BP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5)10097-103
    EDTA plasma (n=4)8984-95














  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected102.7292.7992.7642.59252.3022.3422.3222.1502.51.5131.5871.5501.3781.250.9280.9650.9470.7750.6250.5480.5390.5440.3720.3120.3810.3750.3780.2060.1560.2780.2740.2760.10400.1710.1730.172






  • 货期:3-5 working days

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