首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述:小鼠白介素18结合蛋白(IL-18BP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织培养上清液样本中的IL-18BP含量。IL-18BP(白介素-18结合蛋白)是一种免疫检查点蛋白,可抑制IL-18与其受体结合,调节Th1细胞因子反应。其在多种肿瘤和自身免疫性疾病中高表达,研究机制涉及IL-18与IL-18BP的相互作用及其对免疫反应的影响。试剂盒检测范围为0.156 ng/mL-10 ng/mL,科研人员可将其应用于炎症性疾病动物模型的机制研究、药物干预效果评估或体外细胞实验中IL-18/IL-18BP信号通路的动态监测,特别适用于探索类风湿性关节炎、炎症性肠病等病理过程中IL-18通路的调控机制本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:Il18bp ELISA Kit; Igifbp ELISA Kit; Interleukin-18-binding protein ELISA Kit; IL-18BP ELISA Kit; Interferon gamma-inducing factor-binding protein ELISA Kit
缩写:IL-18BP
Uniprot No.:Q9Z0M9
种属:Mus musculus (Mouse)
样本类型:serum, plasma, cell culture supernates
检测范围:0.156 ng/mL-10 ng/mL
灵敏度:0.039 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Immunology
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-18BP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 97 | ||||
Range % | 93-100 | |||||
1:2 | Average % | 84 | ||||
Range % | 80-88 | |||||
1:4 | Average % | 104 | ||||
Range % | 100-107 | |||||
1:8 | Average % | 91 | ||||
Range % | 87-95 |
回收率:
The recovery of mouse IL-18BP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 100 | 97-103 | ||||
EDTA plasma (n=4) | 89 | 84-95 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
ng/mlOD1OD2AverageCorrected102.7292.7992.7642.59252.3022.3422.3222.1502.51.5131.5871.5501.3781.250.9280.9650.9470.7750.6250.5480.5390.5440.3720.3120.3810.3750.3780.2060.1560.2780.2740.2760.10400.1710.1730.172 |
货期:3-5 working days
×