首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠白介素19(IL19)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL19含量。IL19 属 IL-10 细胞因子家族成员。其在炎症与免疫调节等方面发挥重要作用。研究机制上，它能结合特异性受体，激活下游信号通路，参与调控细胞的增殖、分化以及炎症因子的释放，目前是相关疾病治疗靶点研究的热点。试剂盒检测范围为12.5 pg/mL-800 pg/mL，支持科研领域对小鼠模型中IL19表达动态的研究，例如炎症性疾病机制探索、药物干预效果评估或免疫调控通路分析，为体外实验提供可靠的定量数据支持，但不适用于临床诊断或体内检测用途。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Il19Interleukin-19 ELISA Kit; IL-19 ELISA Kit
缩写：IL19
Uniprot No.：Q8CJ70
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：12.5 pg/mL-800 pg/mL
灵敏度：3.12 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL19 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 107 | |||||
| Range % | 96-112 | ||||||
| 1:2 | Average % | 103 | |||||
| Range % | 94-107 | ||||||
| 1:4 | Average % | 95 | |||||
| Range % | 86-99 | ||||||
| 1:8 | Average % | 100 | |||||
| Range % | 90-104 | ||||||
回收率：
| The recovery of mouse IL19 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 102 | 88-106 | |||||
| EDTA plasma (n=4) | 103 | 92-107 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 8002.7072.6562.6822.542 4001.4941.5441.5191.379 2000.8290.8450.8370.697 1000.4220.4460.4340.294 500.3090.3130.3110.171 250.2010.2120.2070.067 12.50.1760.1710.1740.034 00.1420.1380.140 | |||||||
货期：3-5 working days
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