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产品描述：猪白介素1α(IL-1α)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的IL1A含量。IL1A是重要的细胞因子，在炎症反应等生理病理过程中发挥关键作用。其与多种炎症及免疫相关疾病关联。研究机制上，它主要通过与相应受体结合，激活下游信号通路，从而调节免疫细胞功能、介导炎症反应，是当前热门研究靶点。试剂盒检测范围为25 pg/mL-1600 pg/mL，可为猪类动物模型中的炎症机制研究、疫苗开发评估、疾病进程监测等科研项目提供可靠数据支持，例如在猪繁殖与呼吸综合征、猪流行性腹泻等感染性疾病模型中定量分析IL - 1α的动态变化，或在免疫调控实验中评估细胞因子网络的作用机制。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL1A ELISA Kit; Interleukin-1 alpha ELISA Kit; IL-1 alpha ELISA Kit
缩写：IL1A
Uniprot No.：P18430
种属：Sus scrofa (Pig)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 82-93 | |||||
| 1:2 | Average % | 104 | ||||
| Range % | 100-108 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 87 | ||||
| Range % | 83-92 | |||||
回收率：
| The recovery of pig IL-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 95-103 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected16002.5992.5352.5672.3858001.8391.8991.8691.6874001.1741.1971.1861.0042000.7530.7420.7480.5661000.4600.4990.4800.298500.3680.3720.3700.188250.2840.2920.2880.10600.1810.1830.182 | ||||||
货期：3-5 working days
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