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产品描述：牛白介素1β(IL-1β)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL1B含量。IL-1β（IL1B）是一种重要的促炎细胞因子，在炎症反应、免疫调节等生理和病理过程中起关键作用。研究机制方面，它能激活下游信号通路，引发多种免疫细胞的活化，与多种炎性疾病如类风湿关节炎等的发病机制密切相关，是热门治疗靶点。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，科研人员可将其应用于牛源性疾病模型构建、疫苗免疫效果评估、炎症相关信号通路研究等方向，例如通过检测感染或应激条件下生物样本中IL - 1β的动态变化，揭示免疫调控机制或筛选抗炎药物作用靶点。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL1B ELISA Kit; Interleukin-1 beta ELISA Kit; IL-1 beta ELISA Kit
缩写：IL1B
Uniprot No.：P09428
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 99 | ||||
| Range % | 95-108 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 91-98 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 88-95 | |||||
| 1:8 | Average % | 87 | ||||
| Range % | 81-93 | |||||
回收率：
| The recovery of bovine IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-102 | ||||
| EDTA plasma (n=4) | 92 | 87-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40002.8762.8622.8692.75120002.4442.4392.4422.32410001.9031.8561.8801.7625001.4121.4031.4081.2902501.1641.1851.1751.0571250.8090.7990.8040.68662.50.4710.4790.4750.35700.1170.1180.118 | ||||||
货期：3-5 working days
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