首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒
产品描述：犬白介素1β(IL-1β)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的IL1B含量。IL1B，即白介素-1β，是一种强效的促炎细胞因子，主要由单核细胞、巨噬细胞和树突状细胞产生。它在炎症和免疫反应中起关键作用，通过激活IL-1受体和下游信号通路，调节细胞增殖、分化和凋亡。IL1B的研究对于理解炎症性疾病的发生机制和开发治疗策略具有重要意义。试剂盒检测范围为7.8 pg/mL-500 pg/mL，该产品适用于科研领域，如探究犬类模型中的炎症性疾病病理过程、评估免疫调节剂或抗炎药物的干预效果，以及动物疫苗研发中的免疫应答研究，为兽医学和比较医学领域的实验提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL1BInterleukin-1 beta ELISA Kit; IL-1 beta ELISA Kit
缩写：IL1B
Uniprot No.：Q28292
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 83-100 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 91-99 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-105 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 86-100 | |||||
回收率：
| The recovery of dog IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-102 | ||||
| EDTA plasma (n=4) | 97 | 90-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected5002.5852.6752.6302.5122502.3192.4122.3662.2481251.9571.9721.9651.84762.51.4321.4531.4431.32531.250.7440.7190.7320.61415.630.5170.5080.5130.3957.80.3850.3770.3810.26300.1180.1170.118 | ||||||
货期：3-5 working days
×
