首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述:人白介素1家族成员10(IL1F10)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的IL1F10含量。IL1F10,又称IL-38,是白细胞介素1家族的一员,参与调节适应性及先天免疫反应。其研究背景涉及免疫调节,通过影响细胞因子产生发挥作用,单独不诱导细胞因子产生,但在免疫反应中发挥调节作用。试剂盒检测范围为31.25 pg/mL-2000 pg/mL,适用于基础科研中探索IL1F10在炎症相关疾病模型中的作用机制、评估实验动物或细胞模型的蛋白表达动态变化,以及筛选调控该因子的药物或化合物。科研人员可通过本产品分析不同处理条件下生物样本(如炎症模型血清、肿瘤组织匀浆或免疫细胞培养上清)中IL1F10的表达水平,为免疫调节研究及疾病机理探索提供可靠数据支持。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:family of interleukin 1-theta ELISA Kit; FIL1 theta ELISA Kit; FIL1T ELISA Kit; FKSG75 ELISA Kit; IL 38 ELISA Kit; IL-1 theta ELISA Kit; IL-1F10 (canonical form IL-1F10a) ELISA Kit; IL-1F10 ELISA Kit; IL-1HY2 ELISA Kit; IL1 theta ELISA Kit; IL1F10 ELISA Kit; IL1FA_HUMAN ELISA Kit; IL1HY2 ELISA Kit; Interleukin 1 family member 10 (theta) ELISA Kit; Interleukin 1 receptor antagonist FKSG75 ELISA Kit; Interleukin 1 receptor antagonist homolog 2 ELISA Kit; Interleukin 1 receptor antagonist like FIL1 theta ELISA Kit; Interleukin-1 family member 10 ELISA Kit; Interleukin-1 HY2 ELISA Kit; Interleukin-1 theta ELISA Kit; interleukin-38 ELISA Kit
缩写:IL1F10
Uniprot No.:Q8WWZ1
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates
检测范围:31.25 pg/mL-2000 pg/mL
灵敏度:7.81 pg/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Immunology
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human IL1F10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 97 | ||||
Range % | 92-104 | |||||
1:2 | Average % | 84 | ||||
Range % | 80-88 | |||||
1:4 | Average % | 105 | ||||
Range % | 100-113 | |||||
1:8 | Average % | 95 | ||||
Range % | 90-100 |
回收率:
The recovery of human IL1F10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 88 | 80-92 | ||||
EDTA plasma (n=4) | 94 | 90-99 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
pg/mlOD1OD2AverageCorrected20002.0702.2172.1442.04410001.3931.3211.3571.2575000.7560.8090.7830.6832500.4210.4450.4330.3331250.2670.2540.2610.16162.50.1960.1830.1900.09031.250.1510.1540.1530.05300.1030.0960.100 |
货期:3-5 working days
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