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产品描述：山羊白介素1β(IL-1β)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL1B含量。IL1B即白介素-1β，是重要炎症介质。它在炎症反应、免疫调节等生理病理过程中起关键作用。其研究机制主要围绕它与受体结合后激活下游信号通路，引发炎症级联反应，目前针对它的干预研究有望为炎症相关疾病治疗提供新思路。试剂盒检测范围为3.12 pg/mL-200 pg/mL，适用于动物模型研究、炎症机制探索、免疫调控实验等科研领域，能够为病原体感染评估、炎症因子网络分析、免疫干预效果验证等基础研究提供可靠数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL1BInterleukin-1 beta ELISA Kit; IL-1 beta ELISA Kit
缩写：IL-1β
Uniprot No.：P79162
种属：Capra hircus (Goat)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of goat IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||||||||
| Sample | Serum(n=4) | ||||||||||||
| 1:1 | Average % | 87 | |||||||||||
| Range % | 82-94 | ||||||||||||
| 1:2 | Average % | 90 | |||||||||||
| Range % | 82-97 | ||||||||||||
| 1:4 | Average % | 92 | |||||||||||
| Range % | 85-100 | ||||||||||||
| 1:8 | Average % | 95 | |||||||||||
| Range % | 90-103 | ||||||||||||
回收率：
| The recovery of goat IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||||||||
| Sample Type | Average % Recovery | Range | |||||||||||
| Serum (n=5) | 90 | 85-98 | |||||||||||
| EDTA plasma (n=4) | 99 | 93-108 | |||||||||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||
| pg/mlOD1OD2AverageCorrected2002.1122.0522.0821.9511001.5761.6541.6151.484501.0390.9841.0120.881250.5870.5490.5680.43712.50.3840.3970.3910.2606.250.2920.2690.2810.1503.120.2080.1990.2040.07300.1270.1340.131 | |||||||||||||
货期：3-5 working days
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