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产品描述：鸡白介素1β(IL-1β)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL1B含量。IL1B 即白细胞介素-1β，是一种重要的促炎细胞因子。在炎症反应、免疫调节等生理和病理过程中发挥关键作用。研究机制上，它可激活下游信号通路，引发炎症级联反应，与多种炎症相关疾病如关节炎、心血管病等发病密切相关。试剂盒检测范围为0.27 pg/mL-200 pg/mL，特别适用于家禽免疫学研究、疫苗效力评估、感染性疾病模型建立等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IL-1β
Uniprot No.：O73909
种属：Gallus gallus (Chicken)
样本类型：serum, plasma, tissue homogenates
检测范围：0.27 pg/mL-200 pg/mL
灵敏度：0.27 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 95-106 | |||||
| 1:4 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of chicken IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-98 | ||||
| EDTA plasma (n=4) | 95 | 90-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 2000.1730.1690.171 66.70.2380.2590.249 22.20.3390.3670.353 7.40.4590.4880.474 2.50.6020.6330.618 0.820.8170.8550.836 0.271.0331.0081.021 01.7481.6721.710 | ||||||
货期：3-5 working days
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