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Human Interleukin 1 receptor antagonist, IL-1ra ELISA kit

  • 中文名： 人白介素1受体拮抗剂(IL1Ra)ELISA Kit
货号: IPD-E10396h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人白介素1受体拮抗剂(IL1Ra)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆.样本中的IL1RN含量。IL1RN（白细胞介素1受体拮抗剂）是一种基因，编码的蛋白能抑制IL-1（白细胞介素1）的作用，IL-1在炎症和免疫反应中起重要作用。IL1RN通过调节IL-1的活性，参与维持免疫系统的平衡。试剂盒检测范围为15.6 pg/ml-1000 pg/ml.，灵敏度为3.9 pg/ml。该产品可广泛应用于炎症机制研究、药物开发中抗炎疗法效果评估、疾病模型动物血清标志物分析等科研场景，为探索IL1Ra在免疫调控网络中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    DIRA ELISA Kit; F630041P17Rik ELISA Kit; ICIL 1RA ELISA Kit; ICIL-1RA ELISA Kit; ICIL1RA ELISA Kit; IL-1ra ELISA Kit; IL-1ra3 ELISA Kit; IL-1RN ELISA Kit; IL1 inhibitor ELISA Kit; IL1F3 ELISA Kit; IL1RA ELISA Kit; IL1RA_HUMAN ELISA Kit; IL1RN (IL1F3) ELISA Kit; IL1RN ELISA Kit; Interleukin 1 receptor antagonist ELISA Kit; Interleukin-1 receptor antagonist protein ELISA Kit; Intracellular IL 1 receptor antagonist type II ELISA Kit; Intracellular interleukin 1 receptor antagonist (icIL 1ra) ELISA Kit; IRAP ELISA Kit; MGC10430 ELISA Kit; MVCD4 ELISA Kit; Type II interleukin 1 receptor antagonist ELISA Kit
  • 缩写：
    IL1RN
  • Uniprot No.：
    P18510
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates.
  • 检测范围：
    15.6 pg/ml-1000 pg/ml.
  • 灵敏度：
    3.9 pg/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human IL-1Ra in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    94


    Range %

    85-102


    1:2

    Average %

    90


    Range %

    86-100


    1:4

    Average %

    89


    Range %

    82-95


    1:8

    Average %

    95


    Range %

    87-105


  • 回收率：

    The recovery of human IL-1Ra spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    97

    93-105


    EDTA plasma (n=4)

    100

    94-108


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    1000

    2.503

    2.482

    2.493

    2.413


    500

    1.713

    1.723

    1.718

    1.638


    250

    1.035

    1.017

    1.026

    0.946


    125

    0.604

    0.615

    0.610

    0.530


    62.5

    0.337

    0.319

    0.328

    0.248


    31.25

    0.201

    0.206

    0.204

    0.124


    15.6

    0.154

    0.162

    0.158

    0.078


    0

    0.081

    0.078

    0.080



  • 本试剂盒所含材料：


    • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human IL1RN ANTIBODY. This dismountable microplate can be divided into 12 x 8 strip plates.

    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.

    • One vial Biotin-labeled IL1RN antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.

    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.

    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.

    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.

    • 本试剂盒不含材料：


      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

      • An incubator can provide stable incubation conditions up to 37°C±5°C.

      • Centrifuge

      • Vortex

      • Squirt bottle, manifold dispenser, or automated microplate washer

      • Absorbent paper for blotting the microtiter plate


      • 货期：
        3-5 working days

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